BLASTP 2.0.11 [Jan-20-2000]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ynaE (1307 kb reverse) BIO15.01
         (246 letters)

           457,798 sequences; 140,871,481 total letters

Searching...................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

sp|P50726|YPAA_BACSU  HYPOTHETICAL 20.5 KD PROTEIN IN SERA-F...    69  4e-11
gb|AAD36523.1|AE001797_3  (AE001797) conserved hypothetical ...    46  3e-04
dbj|BAA29323.1|  (AP000001) 191aa long hypothetical protein ...    45  7e-04
emb|CAB49155.1|  (AJ248283) hypothetical protein [Pyrococcus...    43  0.003

 sp|P50726|YPAA_BACSU HYPOTHETICAL 20.5 KD PROTEIN IN SERA-FER INTERGENIC REGION
           >gi|1146197 (L47648) putative [Bacillus subtilis]
           >gi|2634740|emb|CAB14237| (Z99116) ypaA [Bacillus
           subtilis]
           Length = 190
           
 Score = 69.1 bits (166), Expect = 4e-11
 Identities = 47/189 (24%), Positives = 88/189 (45%), Gaps = 17/189 (8%)

Query: 43  KTRRMVLIAMLAALSTXXXXXXXXXXXXXXXDFMKVELSIIPVLIGVFTLGLGDGFIILF 102
           K +++V+++ML++++                D++K++ S +P +I +   G   G  +  
Sbjct: 2   KVKKLVVVSMLSSIA--FVLMLLNFPFPGLPDYLKIDFSDVPAIIAILIYGPLAGIAVEA 59

Query: 103 IRSILWYLLFNQGPSTWIGVPMNFIALGIFMAIVWFFTKKKFSIKNYTVGVVLAAIASVL 162
           I+++L Y++        +G   NFIA  +F+    F  KK  S K   V ++L   A  +
Sbjct: 60  IKNVLQYIIQGSMAGVPVGQVANFIAGTLFILPTAFLFKKLNSAKGLAVSLLLGTAAMTI 119

Query: 163 VMMVLNVFYALPLYRLAAGFDVDKIFVGATHLFNMGSLSVTLNPTYLLTVVLPFNALQYI 222
           +M +LN    LP Y               T   +  +LS +   T ++  +LPFN ++ I
Sbjct: 120 LMSILNYVLILPAY---------------TWFLHSPALSDSALKTAVVAGILPFNMIKGI 164

Query: 223 IFALVFGLI 231
           +  +VF LI
Sbjct: 165 VITVVFSLI 173



 gb|AAD36523.1|AE001797_3 (AE001797) conserved hypothetical protein [Thermotoga maritima]
           Length = 183
           
 Score = 46.1 bits (107), Expect = 3e-04
 Identities = 40/170 (23%), Positives = 75/170 (43%), Gaps = 7/170 (4%)

Query: 41  MSKTRRMVLIAMLAALSTXXXXXXXXXXXXXXXDFMKVELSIIPVLIGVFTLGLGDGFII 100
           MS  +++  + + +AL+T                F+K + S IP LI  F LG G G  +
Sbjct: 1   MSSIKKISFVGIFSALATLVMFLEFPIFPQAS--FLKYDPSEIPALIVSFLLGPGVGMFV 58

Query: 101 LFIRSILWYLLFNQGPSTWIGVPMNFIALGIFMAIVWFFTKKKFSIKNYTVGVVLAAIAS 160
           + ++ IL++L+ +  P   +G+ MN +    F+ I      +  S      G+++A + +
Sbjct: 59  VLVKDILFFLMKSGDP---VGIAMNAVLGMSFVGIAGLIYHRNKSRATAIKGMIVATLFA 115

Query: 161 VLVMMVLNVFYALPLYRLAAGFDVDKIFVGATHLFNMGSLSVTLNPTYLL 210
               + LN    +PLY   A F++   F      FN+    +    T+ +
Sbjct: 116 TAFALGLNAL-IVPLY-FEAPFELYLKFFPFILAFNLVKFGIDSVVTFFV 163



 dbj|BAA29323.1| (AP000001) 191aa long hypothetical protein [Pyrococcus horikoshii]
           Length = 191
           
 Score = 44.9 bits (104), Expect = 7e-04
 Identities = 30/112 (26%), Positives = 57/112 (50%), Gaps = 5/112 (4%)

Query: 76  MKVELSIIPVLIGVFTLGLGDGFIILFIRSILWYLLFNQGPSTW-IGVPMNFIA-LGIFM 133
           M ++L  +P++     LG   GF  L   +I  +++   GP+T  IG  M F A L + +
Sbjct: 33  MNIDLVAVPIIALYLLLGFRAGFFGLITMTIALFII--SGPNTMGIGPIMKFFATLSVLI 90

Query: 134 AI-VWFFTKKKFSIKNYTVGVVLAAIASVLVMMVLNVFYALPLYRLAAGFDV 184
            + +      KF +    +  +L +I   ++M++LN ++ALPL+    G+ +
Sbjct: 91  GLEISGRITNKFGVGYLIIAFILGSIIRDVLMILLNYYFALPLFLKMLGYSI 142



 emb|CAB49155.1| (AJ248283) hypothetical protein [Pyrococcus abyssi]
           Length = 189
           
 Score = 43.0 bits (99), Expect = 0.003
 Identities = 31/114 (27%), Positives = 58/114 (50%), Gaps = 9/114 (7%)

Query: 76  MKVELSIIPVLIGVFTLGLGDGFIILFIRSILWYLLFNQGPSTW-IGVPMNFIALGIFMA 134
           M ++L  +P++     LG   G   L   +I  +++   GP+T  IG  M F A      
Sbjct: 33  MNIDLVAVPIIALYLILGFRAGLFGLAAMTIGLFII--SGPNTMGIGPVMKFFAT--LSV 88

Query: 135 IVWFFTKK----KFSIKNYTVGVVLAAIASVLVMMVLNVFYALPLYRLAAGFDV 184
           ++   T K    K S+  + V  ++AA+   ++M++LN ++ALPL+    G+++
Sbjct: 89  LIGLETSKAIIKKKSVTYFIVAFLVAAVIRDVLMILLNYYFALPLFLKMMGYNI 142



    Posted date:  Mar 2, 2000 12:24 PM
  Number of letters in database: 140,871,481
  Number of sequences in database:  457,798
  
Lambda     K      H
   0.335    0.148    0.454 

Gapped
Lambda     K      H
   0.270   0.0470    0.230 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55618880
Number of Sequences: 457798
Number of extensions: 2067667
Number of successful extensions: 12099
Number of sequences better than 1.0e-02: 4
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 12096
Number of HSP's gapped (non-prelim): 4
length of query: 246
length of database: 140,871,481
effective HSP length: 48
effective length of query: 198
effective length of database: 118,897,177
effective search space: 23541641046
effective search space used: 23541641046
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 39 (21.6 bits)
S2: 95 (41.4 bits)